Journal: EMBO Reports
Article Title: Interferon‐induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20
doi: 10.15252/embr.201949568
Figure Lengend Snippet: Liver tissue samples obtained from HBV‐negative patients undergoing metastasis resection (control) or patients with acute, self‐limiting HBV infection or chronic hepatitis B, or chronic coinfection with HBV and hepatitis D virus (HDV) were stained for ISG20 by immunohistochemistry. For each clinical entity, tissue sections from three different patients are shown; scale bar: 50 µm. ISG20‐positive area of each sample ( n = 3 biological replicates) was determined by Tissue IA image analysis software and is given in % of total tissue area scanned. Data information: Data are represented as mean ± SD. ** P < 0.01 and ns: not significant by one‐way ANOVA.
Article Snippet: For quantification, image analysis software Tissue IA (Slidepath, Leica, Wetzlar, Germany) was used with optimized color detection and quantification algorithms at 40× magnification.
Techniques: Control, Infection, Virus, Staining, Immunohistochemistry, Software